Alpha-Factor Mating Pheromone Epigenetics High-Throughput/Automated Isolation SDS-PAGE and other sensitive applications

SDS-PAGE and other sensitive applications

Mild expression of a chromosomally encoded bacteriophage λ R gene

Simply choose a desired cutoff to bind the target of interest onto the beads and remove species outside of this range

For automation scripts and support

and recovering a sample from the column

Alpha-Factor Mating Pheromone Epigenetics High-Throughput/Automated Isolation SDS-PAGE and other sensitive applicationsWhen yeast "a" and "alpha" cells encounter mating pheromones of the opposite cell type they induce genes necessary for mating, arrest the cell cycle in G1, alter cell surface and nuclear determinants, and also undergo dramatic morphological elongation into pear shapes, affectionately termed "schmooing". These alterations prepare the yeast cells for mating and fusion to form stable diploids. The a alpha diploids are not responsive to mating pheromone