Methylated & Non-methylated pUC19 DNA Set Chromatin Analysis Yeast prepared with this kit
Description
Yeast prepared with this kit can be transformed with both circular and linear DNAs
Simply add DNA/RNA Shield and Proteinase K to extract total RNA from any tissue
DNA/RNA is eluted into ≥50 µl of DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing
coli in only 15 minutes
The extracted DNA is suitable for immediate use in sensitive downstream applications including qPCR
Methylated & Non-methylated pUC19 DNA Set Chromatin Analysis Yeast prepared with this kitThe Methylated & Non methylated pUC19 DNA Set consists of control DNAs and a set of specifically designed primers. The set is ideal as a spike in control to assess bisulfite conversion efficiency within the same reaction as the sample, or to produce known mixtures of methylated and non methylated DNA for assay calibration. The non methylated pUC19 DNA is pUC19 isolated from a methylation negative strain of bacteria (Dam, Dcm), and the methylated pUC19
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