RRHP 5-hmC Library Prep Kit Name:RRHP 5-hmC Library Prep Kit 7 ml (dry volume) mixed

7 ml (dry volume) mixed 0

total RNA from any fungus that can be lysed by yeast lytic enzyme

This magnetic bead-based bisulfite conversion kit produces the highest yields of bisulfite-converted DNA that is ideal for PCR

wash and elute

RNA is eluted into ≥ 6 µl of RNase-free water and is ready for any downstream application including RT-PCR

RRHP 5-hmC Library Prep Kit Name:RRHP 5-hmC Library Prep Kit 7 ml (dry volume) mixedThe RRHP 5 hmC Library Prep Kit is an all inclusive solution for analysis of genome wide 5 hydroxymethylcytosine (5 hmC) positions at single base resolution. The Reduced Representation Hydroxymethylation Profiling (RRHP) method is based on blocking MspI digestion by glucosylating 5 hmC within MspI recognition sites. Fragments lacking glucosylated 5 hmC at the adapter ligation junction will be cleaved and not amplified by PCR. Therefore, only fragments