5-Fluoroorotic Acid (5-FOA) High-Throughput/Automated Isolation soft tissues

soft tissues

R1014) RNA

Each marker comes with product information detailing the product and its application

Used in the EZ Nucleosomal DNA Prep Kit to stop micrococcal nuclease reaction and prepare for nucleosomal DNA purification

DNA/RNA is washed and concentrated into a small volume of water

5-Fluoroorotic Acid (5-FOA) High-Throughput/Automated Isolation soft tissuesUsing 5 Fluoroorotic Acid (5 FOA) for the counter selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two hybrid screens are methods that can employ the use of 5 FOA. Otherwise nontoxic to yeast, 5 FOA is converted to the toxic form (i. e., 5 flurouracil) in strains expressing the functional URA3 gene coding for orotine 5 monophosphate decarboxylase that is involved in